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Looks more like a greenhouse frog rather than coqui. Very cute. https://mauiinvasive.org/coqui-frog-identification/

Should be careful what you wish for. Seems like it could be one of these. https://wildlife.ca.gov/Conservation/Invasives/Species/Coqui

I've been on vacation for a few days. Will be working on labs next week.

I'll work on the details of what the ideal sample will be. You are correct Terry. We will likely need a small piece of mature leaf that isn't actively growing to minimize capturing a lot of cells with duplicating chromosomes. Orchids grow slowly, so even in such a case it probably would change...

Spot on. I'm super curious what we will find. I'm also speaking with my friend at Illumina. They are the biggest gorilla in next generation sequencing. There may be additional data we could capture in parallel depending on the companies that do plant genetics/genomics since we will be gathering...

Terry, I agree that this would (hopefully) be most useful for telling if a plant is diploid or tretaploid and is likely the target species or not. There is some potential for people to have plants that are triploids (as a result of breeding 2n x 4n plants). This case would potentially require...

Thanks for the contribution Ed. I think we will likely need to set aside the question of species of hybrid origin or that are actually a complex mixture of genomes on their way to figuring out if it becomes one species over time, a species complex or stays two distinct species. This is at the...

Working on that now. There are quite a few labs that do this for other crop species and ornamental plants. Just a matter of figuring out who will deliver reliable data generation, be a good repository and the cost. This is where the start small and possible test a couple of labs might be the way...

This is great. Please see Cassio van den Berg's thesis http://cassiovandenberg.yolasite.com/resources/thesis.pdf starting on page 51 for chromosome counts. The baseline chromosome count for the almost the entirety of the Laeliinae is 2n = 40. When you see number that are +/- keep in mind these...

I've spoken to a few of my other orchid friends, so I'm pretty sure we can figure out getting access to a great pool of plants. The first key will be to try and nail down either divisions preferably or mericlones of know or very high probability wild source material of a few species that are...

Found a nice paper to get the thinking going. A couple of years old, but that just means the cost has dropped even further. A guide to sequence your favorite plant genomes At this point sequencing a genome in itself isn't the cost driver. It's the human time to analyze the data and make...

Hi Leslie, Thanks for contibuting! My thoughts are perhaps along the lines of the lean startup methodology of developing the minimum viable product and then iterate and add complexity once we have proof of principle. I think we could have some short term goals in the pursuit of a grander goal...

Hi Terry, The short answer is yes. I'm beginning to wonder if there is an even lower tech solution than fishing out genes for at least a first level screening. People are already using flow cytometry to just look at total nuclear DNA content to evaluate if plants are diploid, triploid and...

Willing to help hedge your bets of disaster by growing some of them for you. 😇

I have a soft spot for mendelii and rex. Both should be much more widely grown. Beautiful.

I'll wait until one of the tipos is in bloom and do a side by side.

https://www.slippertalk.com/threads/proposal-to-evaluate-genetic-tools-to-evaluate-cattleya-species.51260/

New thread based on Is this jenmanii alba? discussion that developed out of is this plant Cattleya jenmanii or not? The idea behind this thread is to think about how to use our collections to crowdsource plant material and financial support to pilot an evaluation of using modern genetic...

This is very cool how intersted people are. I will set up another thread shortly. I second Terry's idea of picking one or a couple of species to think through how to do something like this on a small scale and show it can be done and then how it could be expanded.

Besides traditional farmers that are doing this for cash crops, the biggest driver appears to be marijuana growers. I think this might be the key as there are a number of testing services that are starting to combine genotypic and phenotypic screening for a price sensitive group that isn't...