In vitro propagation of Calypso bulbosa

[Berthold]

Six month after sowing

reaching the second summer rest period ex vitro and is ready to flower next spring

 

[NYEric]

Excellent, keep us posted.

 

[Brian Monk]

Congrats!!!! Cold you elaborate on the media you flasked onto? Did you replate at all? Did you use sucrose, glucose, fructose?? What about auxins vs. giberellins? Very nice, glad to see someone working hard with these species!!

 

[cnycharles]

nice. are these var. occidentalis or americanus (western or eastern)?

 

[biothanasis]

Exciting... :clap: :clap:

 

[NYEric]

Oh I forgot to add, I'll take 5!

 

[Berthold]

nice. are these var. occidentalis or americanus (western or eastern)?

its the western variation, seeds from California.

Left the baby from the thread starter post, right an adult plant with a bloom shoot.
Sorry, no bloom of the baby in 2012 but 2013 I hope.

The Calypso babies are very hand made.

 

[SlipperFan]

I don't remember seeing a Calypso posted here before.

 

[cnycharles]

i've never seen one in the wild yet, though wandered around some spots in vermont looking, with not very good directions I might add . hope you get some flowers!

 

[NYEric]

Thanks for sharing. Just curious but; how many survived from test tube?

 

[Berthold]

Thanks for sharing. Just curious but; how many survived from test tube?

From one tube I could keep alive 5 seedlings.

But in the tubes from Claus under sterile condition the the seedlings started multiplying. The plantlets can be seperated and develope to complete plants in the tube.

 

[Dido]

great job done from you and claus.

The invitro bulbs looks great at claus. Hope he will be succesfull to deflask them.

 

[paphioboy]

Great ex-situ 'conservation' effort of this interesting (and rare?) species...

 

[biothanasis]

Great effort!!! Well done!!!

 

[NYEric]

Thanks for the info. Good luck.

 

[Berthold]

The seedling yet still decided to bloom in the coming season, maybe march I hope.

That was the beginning

6 month after sowing

After 8 month

10 weeks after deflasking into so called Neudohum substrate

 

[SlipperFan]

Looking good!

 

[biothanasis]

nice work!!! Can't wait to see it blooming...

 

[tocarmar]

What care do you give them for winter???

 

[Berthold]

I keep the pots in the greenhouse at about 5° C.

Outdoors in Germany is also possible but I would have a higher risc due to rotting in warm and wet winters and by snails and insects.